Anti-GRHL2 Antibody Picoband®

SKU: A04120-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GRHL2

Specificity:

Background: The protein encoded by this gene is a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28).

Immunogen: E.coli-derived human GRHL2 recombinant protein (Position: M1-I625).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chen, W., Dong, Q., Shin, K.-H., Kim, R. H., Oh, J.-E., Park, N.-H., Kang, M. K. Grainyhead-like 2 enhances the human telomerase reverse transcriptase gene expression by inhibiting DNA methylation at the 5-prime-CpG island in normal human keratinocytes. J. Biol. Chem. 285: 40852-40863, 2010. 2. Han, Y., Mu, Y., Li, X., Xu, P., Tong, J., Liu, Z., Ma, T., Zeng, G., Yang, S., Du, J., Meng, A. Grhl2 deficiency impairs otic development and hearing ability in a zebrafish model of the progressive dominant hearing loss DFNA28. Hum. Molec. Genet. 20: 3213-3226, 2011. 3. Jacobs, J., Atkins, M., Davie, K., Imrichova, H., Romanelli, L., Christiaens, V., Hulselmans, G., Potier, D., Wouters, J., Taskiran, I. I., Paciello, G., Gonzalez-Blas, C. B., Koldere, D., Aibar, S., Halder, G., Aerts, S. The transcription factor grainy head primes epithelial enhancers for spatiotemporal activation by displacing nucleosomes. Nature Genet. 50: 1011-1020, 2018.

Uniprot ID: Q6ISB3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 71 kDa

Calculated Molecular Weight: 211344 MW

Gene ID: 79977

Protein Name: Grainyhead-like protein 2 homolog

Gene Full Name: grainyhead like transcription factor 2

Synonyms: GRHL2; BOM; TFCP2L3; Grainyhead-like protein 2 homolog; Brother of mammalian grainyhead; Transcription factor CP2-like 3