Anti-GFAP Antibody (Monoclonal, G-A-5)

SKU: MA1045

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Pig,Rat

Applications: IF,IHC,IHC-F,WB

Application Details: Western blot, 0.5-1μg/ml, Human, mouse, pig, rat
Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, pig, rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, pig, rat, -
Immunofluorescence, 2μg/ml, Rat

Gene Name: GFAP

Specificity: No cross reactivity with other proteins.

Background: Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.

Immunogen: GFAP from pig spinal cord.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Brenner, M.; Johnson, A. B.; Boespflug-Tanguy, O.; Rodriguez, D.; Goldman, J. E.; Messing, A. : Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nature Genet. 27: 117-120, 2001. 2. Rodriguez, D.; Gauthier, F.; Bertini, E.; Bugiani, M.; Brenner, M.; N'guyen, S.; Goizet, C.; Gelot, A.; Surtees, R.; Pedespan, J.-M.; Hernandorena, X.; Troncoso, M.; Uziel, G.; Messing, A.; Ponsot, G.; Pham-Dinh, D.; Dautigny, A.; Boespflug-Tanguy, O. : Infantile Alexander disease: spectrum of GFAP mutations and genotype-phenotype correlation. Am. J. Hum. Genet. 69: 1134-1140, 2001. Note: Erratum: Am. J. Hum. Genet. 69: 1413 only, 2001.

Uniprot ID: P47819

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: G-A-5

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight: 49957 MW

Gene ID: Human(2670), Mouse(14580), Rat(24387)

Protein Name: Glial fibrillary acidic protein

Gene Full Name: glial fibrillary acidic protein

Synonyms: FLJ45472; intermediate filament protein