Anti-GAD65/GAD2 Antibody Picoband® (monoclonal, 4E12)

SKU: M03142-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Mouse, Rat

Gene Name: GAD2

Specificity:

Background: Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65, different from the related mouse and rat sequences by one amino acid.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: GAD2 glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa). 2. Meyre, D., Boutin, P., Tounian, A., Deweirder, M., Aout, M., Jouret, B., Heude, B., Weill, J., Tauber, M., Tounian, P., Froguel, P. Is glutamate decarboxylase 2 (GAD2) a genetic link between low birth weight and subsequent development of obesity in children?J. Clin. Endocr. Metab. 90: 2384-2390, 2005. 3. Zhang, Z., Cai, Y. Q., Zou, F., Bie, B., Pan, Z. Z. Epigenetic suppression of GAD65 expression mediates persistent pain. Nature Med. 17: 1448-1455, 2011.

Uniprot ID: Q05329

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: 4E12

Observed Molecular Weight: 65 kDa

Calculated Molecular Weight:

Gene ID: 2572

Protein Name: Glutamate decarboxylase 2

Gene Full Name: glutamate decarboxylase 2

Synonyms: GAD 65; GAD2; GAD65; Glutamate decarboxylase 2