Anti-GABA A Receptor alpha 1/GABRA1 Antibody Picoband®

SKU: PA1578

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Gene Name: GABRA1

Specificity: No cross reactivity with other proteins.

Background: GABRA1, Gamma-aminobutyric acid receptor subunit alpha-1, is a protein that in humans is encoded by the GABRA1 gene. The 1,055-bp GABRA1 clone contained an open reading frame and 260 nucleotides in the 5-prime noncoding region. The 351-amino acid sequence shows 97% homology with its bovine counterpart. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GABA A Receptor alpha 1, identical to the related rat and mouse sequences.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Cossette, P., Liu, L., Brisebois, K., Dong, H., Lortie, A., Vanasse, M., Saint-Hilaire, J.-M., Carmant, L., Verner, A., Lu, W.-Y., Wang, Y. T., Rouleau, G. A.Mutation of GABRA1 in an autosomal dominant form of juvenile myoclonic epilepsy.Nature Genet. 31: 184-189, 2002. 2. Kralic, J. E., Criswell, H. E., Osterman, J. L., O'Buckley, T. K., Wilkie, M. E., Matthews, D. B., Hamre, K., Breese, G. R., Homanics, G. E., Morrow, A. L.Genetic essential tremor in gamma-aminobutyric acid-A receptor alpha-1 subunit knockout mice.J. Clin. Invest. 115: 774-779, 2005. 3. Rudolph, U., Crestani, F., Benke, D., Brunig, I., Benson, J. A., Fritschy, J.-M., Martin, J. R., Bluethmann, H., Mohler, H.Benzodiazepine actions mediated by specific gamma-aminobutyric acid-A receptor subtypes.Nature 401: 796-800, 1999.

Uniprot ID: P14867

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 52 kDa

Calculated Molecular Weight: 51802 MW

Gene ID: 2554

Protein Name: Gamma-aminobutyric acid receptor subunit alpha-1

Gene Full Name: gamma-aminobutyric acid type A receptor subunit alpha1

Synonyms: ECA4; EJM; GABA A receptor alpha 1; GABAA receptor alpha 1; GABRA1