Anti-FYCO1 Antibody Picoband®

SKU: A06691-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: FYCO1

Specificity:

Background: The gene encodes a Rab7 adapter protein that is implicated in the microtubule transport of autophagosomes. The encoded protein contains a RUN domain, a FYVE-type zinc finger domain, and Golgi dynamics (GOLD) domain. The encoded protein plays a role in microtubule plus end-directed transport of autophagic vesicles through interactions with the small GTPase Rab7, phosphatidylinositol-3-phosphate (PI3P), the autophagosome marker LC3, and the kinesin KIF5. Mutations in this gene are associated with inclusion body myositis (IBM) and autosomal recessive congenital cataracts (CATC2).

Immunogen: E.coli-derived human FYCO1 recombinant protein (Position: M1-K1019). Human FYCO1 shares 72.2% amino acid (aa) sequence identity with mouse FYCO1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chen, J., Ma, Z., Jiao, X., Fariss, R., Kantorow, W. L., Kantorow, M., Pras, E., Frydman, M., Pras, E., Riazuddin, S., Riazuddin, S. A., Hejtmancik, J. F. Mutations in FYCO1 cause autosomal-recessive congenital cataracts. Am. J. Hum. Genet. 88: 827-838, 2011. 2. Kiss, H., Yang, Y., Kiss, C., Andersson, K., Klein, G., Imreh, S., Dumanski, J. P. The transcriptional map of the common eliminated region 1 (C3CER1) in 3p21.3. Europ. J. Hum. Genet. 10: 52-61, 2002. 3. Pras, E., Pras, E., Bakhan, T., Levy-Nissenbaum, E., Lahat, H., Assia, E. I., Garzozi, H. J., Kastner, D. L., Goldman, B., Frydman, M. A gene causing autosomal recessive cataract maps to the short arm of chromosome 3. Isr. Med. Assoc. J. 3: 559-562, 2001.

Uniprot ID: Q9BQS8

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 167 kDa

Calculated Molecular Weight: 167 kDa

Gene ID: 79443

Protein Name: FYVE and coiled-coil domain-containing protein 1

Gene Full Name: FYVE and coiled-coil domain autophagy adaptor 1

Synonyms: FYCO1; RUFY3; ZFYVE7