Anti-Filaggrin/FLG Antibody Picoband®

SKU: PB9870

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human

Gene Name: FLG

Specificity: No cross reactivity with other proteins.

Background: In humans, profillagrin is encoded by the?FLG?gene, which is part of the S100 fused-type protein (SFTP) family within the?epidermal differentiation complex?on?chromosome 1q21. The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.

Immunogen: E. coli-derived human Filaggrin recombinant protein (Position: M1-R261).

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Kypriotou, Magdalini; Huber, Marcel; Hohl, Daniel (September 2012).The human epidermal differentiation complex: cornified envelope precursors, S100 proteins and the ‘fused genes’ family. Experimental Dermatology 21 (9): 643–649. 2. Markova, NG; Marekov, LN; Chipev, CC; Gan, SQ; Idler, WW; Steinert, PM (January 1993). Profilaggrin is a major epidermal calcium-binding protein.. Molecular and Cellular Biology 13 (1): 613–25.

Uniprot ID: P20930

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 435 kDa

Calculated Molecular Weight: 435170 MW

Gene ID: 2312

Protein Name: Filaggrin

Gene Full Name: filaggrin

Synonyms: Filaggrin; FLG