Anti-FASL/FASLG Antibody Picoband®

SKU: A00925-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: FASLG

Specificity:

Background: This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human FASL/FASLG, which shares 85% and 80% amino acid (aa) sequence identity with mouse and rat FASL/FASLG, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Asanuma, K., Tsuji, N., Endoh, T., Yagihashi, A., Watanabe, N. Survivin enhances Fas ligand expression via up-regulation of specificity protein 1-mediated gene transcription in colon cancer cells. J. Immun. 172: 3922-3929, 2004. 2. Asanuma, K., Tsuji, N., Endoh, T., Yagihashi, A., Watanabe, N. Survivin enhances Fas ligand expression via up-regulation of specificity protein 1-mediated gene transcription in colon cancer cells. J. Immun. 172: 3922-3929, 2004. 3. Borges, V. M., Falcao, H., Leite-Junior, J. H., Alvim, L., Teixeira, G. P., Russo, M., Nobrega, A. F., Lopes, M. F., Rocco, P. M., Davidson, W. F., Linden, R., Yagita, H., Zin, W. A., DosReis, G. A. Fas ligand triggers pulmonary silicosis. J. Exp. Med. 194: 155-163, 2001.

Uniprot ID: P48023

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 31 kDa

Calculated Molecular Weight: 31 kDa

Gene ID: 356

Protein Name: Tumor necrosis factor ligand superfamily member 6

Gene Full Name: Fas ligand

Synonyms: Apoptosis antigen ligand; APT1LG1; APTL; CD178; CD95 L; CD95 ligand; CD95L; Fas antigen ligand; Fas ligand; FASL; FASLG; TNFSF6