Anti-FADS1 Antibody Picoband®

SKU: A02535-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: FADS1

Specificity:

Background: Fatty acid desaturase 1 is an enzyme that in humans is encoded by the FADS1 gene. The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.

Immunogen: E.coli-derived human FADS1 recombinant protein (Position: M1-M257).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Ameur, A., Enroth, S., Johansson, A., Zaboli, G., Igl, W., Johansson, A. C. V., Rivas, M. A., Daly, M. J., Schmitz, G., Hicks, A. A., Meitinger, T., Feuk, L., van Duijn, C., Oostra, B., Pramstaller, P. P., Rudan, I., Wright, A. F., Wilson, J. F., Campbell, H., Gyllensten, U. Genetic adaptation of fatty-acid metabolism: a human-specific haplotype increasing the biosynthesis of long-chain omega-3 and omega-6 fatty acids. Am. J. Hum. Genet. 90: 809-820, 2012. 2. Cho, H. P., Nakamura, M., Clarke, S. D. Cloning, expression, and fatty acid regulation of the human delta-5 desaturase. J. Biol. Chem. 274: 37335-37339, 1999. 3. Marquardt, A., Stohr, H., White, K., Weber, B. H. F. cDNA cloning, genomic structure, and chromosomal localization of three members of the human fatty acid desaturase family. Genomics 66: 175-183, 2000.

Uniprot ID: O60427

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 52 kDa

Calculated Molecular Weight: 87164 MW

Gene ID: 3992

Protein Name: Acyl-CoA

Gene Full Name: fatty acid desaturase 1

Synonyms: D5D; Delta(5) desaturase; Delta(5) fatty acid desaturase; FADS1; FADS6; FADSD5; fatty acid desaturase 1; LLCDL1; TU12