Anti-FADD Antibody Picoband®

SKU: PA1464

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: FADD

Specificity: No cross reactivity with other proteins.

Background: FADD, Fas-Associated protein with Death Domain, is a universal adaptor protein in apoptosis that mediates signaling of all known death domain-containing members of the TNF receptor superfamily. The FADD gene contains 2 exons and spans approximately 3.6 kb. By analysis of somatic cell hybrid panels and by fluorescence in situ hybridization, the FADD gene is mapped to 11q13.3. The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, thus, it participates in the death signaling initiated by these receptors.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human FADD.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Kabra, N. H., Kang, C., Hsing, L. C., Zhang, J., Winoto, A.T cell-specific FADD-deficient mice: FADD is required for early T cell development.Proc. Nat. Acad. Sci. 98: 6307-6312, 2001. 2. Kim, P. K. M., Dutra, A. S., Chandrasekharappa, S. C., Puck, J. M.Genomic structure and mapping of human FADD, an intracellular mediator of lymphocyte apoptosis.J. Immun. 157: 5461-5466, 1996. 3. Lee, H.-J., Pyo, J.-O., Oh, Y., Kim, H.-J., Hong, S., Jeon, Y.-J., Kim, H., Cho, D.-H., Woo, H.-N., Song, S., Nam, J.-H., Kim, H. J., Kim, K.-S., Jung, Y.-K.AK2 activates a novel apoptotic pathway through formation of a complex with FADD and caspase-10.Nature Cell Biol. 9: 1303-1310, 2007.

Uniprot ID: Q13158

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 54 kDa

Calculated Molecular Weight: 23279 MW

Gene ID: 8772

Protein Name: FAS-associated death domain protein

Gene Full Name: Fas associated via death domain

Synonyms: FADD; GIG3; MORT1; Protein FADD