Anti-EVI1/MECOM Antibody Picoband®

SKU: A04590-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MECOM

Specificity:

Background: MDS1 and EVI1 complex locus protein EVI1 (MECOM) also known as ecotropic virus integration site 1 protein homolog (EVI-1) or positive regulatory domain zinc finger protein 3 (PRDM3) is a protein that in humans is encoded by the MECOM gene. The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene.

Immunogen: E.coli-derived human EVI1/MECOM recombinant protein (Position: E125-D743).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Buonamici, S., Li, D., Chi, Y., Zhao, R., Wang, X., Brace, L., Ni, H., Saunthararajah, Y., Nucifora, G. EVI1 induces myelodysplastic syndrome in mice. J. Clin. Invest. 114: 713-719, 2004. Note: Erratum: J. Clin. Invest. 115: 2296 only, 2005. 2. Gross, M. B. Personal Communication. Baltimore, Md. 3/16/2018. 3. Morishita, K., Parganas, E., Bartholomew, C., Sacchi, N., Valentine, M. B., Raimondi, S. C., Le Beau, M. M., Ihle, J. N. The human Evi-1 gene is located on chromosome 3q24-q28 but is not rearranged in three cases of acute nonlymphocytic leukemias containing t(3;5)(q25;q34) translocations. Oncogene Res. 5: 221-231, 1990.

Uniprot ID: Q03112

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 138 kDa

Calculated Molecular Weight:

Gene ID: 2122

Protein Name: Histone-lysine N-methyltransferase MECOM

Gene Full Name: MDS1 and EVI1 complex locus

Synonyms: AML1 EVI 1; EVI 1; EVI1; MDS1 EVI1; MECOM; PRDM3