Anti-ERCC1 Antibody Picoband®

SKU: A00388-4

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ERCC1

Specificity:

Background: DNA excision repair protein ERCC-1 is a protein that in humans is encoded by the ERCC1 gene. The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Immunogen: E.coli-derived human ERCC1 recombinant protein (Position: K7-R283).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Imoto, K., Boyle, J., Oh, K., Khan, S., Ueda, T., Nadem, C., Slor, H., Orgal, S., Gadoth, N., Busch, D., Jaspers, N. G., Tamura, D., DiGiovanna, J. J., Kraemer, K. H. Patients with defects in the interacting nucleotide excision repair proteins ERCC1 or XPF show xeroderma pigmentosum with late onset severe neurological degeneration. (Abstract) J. Invest. Derm. 127: S92 only, 2007. 2. McCabe, K. M., Hemphill, A., Akkari, Y., Jakobs, P. M., Pauw, D., Olson, S. B., Moses, R. E., Grompe, M. ERCC1 is required for FANCD2 focus formation. Molec. Genet. Metab. 95: 66-73, 2008.

Uniprot ID: P07992

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight: 17628 MW

Gene ID: 2067

Protein Name: DNA excision repair protein ERCC-1

Gene Full Name: ERCC excision repair 1, endonuclease non-catalytic subunit

Synonyms: COFS4; ERCC1; UV20