Anti-eIF3B Antibody Picoband®

SKU: PA2030

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: EIF3B

Specificity: No cross reactivity with other proteins.

Background: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B), also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al. (1997) identified a cDNA encoding a human Prt1 homolog. Asano et al. (1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of 2 proteins, p116 (PRT1) and an unrelated 110-kD protein. Chaudhuri et al. (1997) isolated cDNAs encoding PRT1, which they called p110 based on the size of the corresponding component of rabbit eIF3.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human eIF3B, different from the related rat and mouse sequences by one amino acid.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Asano, K., Kinzy, T. G., Merrick, W. C., Hershey, J. W. B. Conservation and diversity of eukaryotic translation initiation factor eIF3. J. Biol. Chem. 272: 1101-1109, 1997. 2. Chaudhuri, J., Chakrabarti, A., Maitra, U. Biochemical characterization of mammalian translation initiation factor 3 (eIF3): molecular cloning reveals that p110 subunit is the mammalian homologue of saccharomyces cerevisiae protein Prt1. J. Biol. Chem. 272: 30975-30983, 1997. 3. Methot, N., Rom, E., Olsen, H., Sonenberg, N. The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170. J. Biol. Chem. 272: 1110-1116, 1997.

Uniprot ID: P55884

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 115 kDa

Calculated Molecular Weight: 92482 MW

Gene ID: 8662

Protein Name: Eukaryotic translation initiation factor 3 subunit B

Gene Full Name: eukaryotic translation initiation factor 3 subunit B

Synonyms: eIF 3 eta; EIF3 ETA; EIF3 P110; EIF3 P116; EIF3B; EIF3S9; hPrt1; PRT1; Prt1 homolog