Anti-EHD2 Antibody Picoband®

SKU: A04265-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IF,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human

Gene Name: EHD2

Specificity:

Background: EH-domain containing 2, also known as EHD2, is a human gene[5] belonging to the EHD protein family. This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human EHD2, which shares 100% and 95% amino acid (aa) sequence identity with mouse and rat EHD2, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Daumke, O., Lundmark, R., Vallis, Y., Martens, S., Butler, P. J. G., McMahon, H. T. Architectural and mechanistic insights into an EHD ATPase involved in membrane remodelling. Nature 449: 923-927, 2007. 2. Guilherme, A., Soriano, N. A., Bose, S., Holik, J., Bose, A., Pomerleau, D. P., Furcinitti, P., Leszyk, J., Corvera, S., Czech, M. P. EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton. J. Biol. Chem. 279: 10593-10605, 2004. 3. Pohl, U., Smith, J. S., Tachibana, I., Ueki, K., Lee, H. K., Ramaswamy, S., Wu, Q., Mohrenweiser, H. W., Jenkins, R. B., Louis, D. N. EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins. Genomics 63: 255-262, 2000.

Uniprot ID: Q9NZN4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 61 kDa

Calculated Molecular Weight:

Gene ID: 30846

Protein Name: EH domain-containing protein 2

Gene Full Name: EH domain containing 2

Synonyms: EH domain containing 2; EH domain containing protein 2; EHD2; PAST homolog 2; PAST2