Anti-DDX1 Picoband® Antibody (monoclonal, 2D4)

SKU: M03727-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: DDX1

Specificity:

Background: ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene. It is mapped to 2p24.3. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.

Immunogen: E.coli-derived human DDX1 recombinant protein (Position: K562-F740).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Chen HC, Lin WC, Tsay YG, Lee SC, Chang CJ (October 2002). An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K. The Journal of Biological Chemistry. 277 (43): 40403–9. 2. Cho WJ, Yoon WJ, Moon CH, Cha SJ, Song H, Cho HR, Jang SJ, Chung DK, Jeong CS, Park JW (November 2002). Molecular cloning of a novel chaperone-like protein induced by rhabdovirus infection with sequence similarity to the bacterial extracellular solute-binding protein family 5. The Journal of Biological Chemistry. 277 (44): 41489–96. 3. Li L, Li HS, Pauza CD, Bukrinsky M, Zhao RY (2006). Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions. Cell Research. 15 (11–12): 923–34.

Uniprot ID: Q92499

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2a

Phospho_site:

Clone Number: Clone: 2D4

Observed Molecular Weight: 86 kDa

Calculated Molecular Weight:

Gene ID: 1653

Protein Name: ATP-dependent RNA helicase DDX1

Gene Full Name: DEAD-box helicase 1

Synonyms: DBP RB; DDX1; DEAD box protein 1; UKVH5d