Anti-Cytokeratin 4/KRT4 Antibody Picoband®

SKU: A07410-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: KRT4

Specificity:

Background: Keratin, type I cytoskeletal 4 also known as cytokeratin-4 (CK-4) or keratin-4 (K4) is a protein that in humans is encoded by the KRT4 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Immunogen: E.coli-derived human Cytokeratin 4/KRT4 recombinant protein (Position: N170-S427).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Barletta, C., Batticane, N., Ragusa, R. M., Leube, R., Peschle, C., Romano, V. Subchromosomal localization of two human cytokeratin genes (KRT4 and KRT15) by in situ hybridization. Cytogenet. Cell Genet. 54: 148-150, 1990. 2. Barletta, C., Batticane, N., Ragusa, R. M., Leube, R. E., Franke, W. W., Peschle, C., Romano, V. Sub-chromosomal localization of human cytokeratin 4, 15, and 19 genes. (Abstract) Cytogenet. Cell Genet. 51: 958 only, 1989. 3. Chao, S.-C., Tsai, Y.-M., Yang, M.-H., Lee, J. Y.-Y. A novel mutation in the keratin 4 gene causing white sponge naevus. Brit. J. Derm. 148: 1125-1128, 2003.

Uniprot ID: P19013

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 56 kDa

Calculated Molecular Weight: 51200 MW

Gene ID: 3851

Protein Name: Keratin, type II cytoskeletal 4

Gene Full Name: keratin 4

Synonyms: CK 4; CK4; CYK4; Cytokeratin 4; K4; keratin 4; KRT4; Type II keratin Kb4