Anti-CYP51A1/CYP51 Antibody Picoband®

SKU: A04032-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Monkey

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Monkey
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: CYP51A1

Specificity:

Background: P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. CYP51A1 (cytochrome P450, family 51, subfamily A, polypeptide 1), also known as LDM (lanosterol 14-α demethylase) or cytochrome P450-14DM, is a 503 amino acid protein localized to endoplasmic reticulum membrane. CYP51A1 is an important enzyme for zymosterol and steroid biosynthesis. CYP51A1 catalyzes C14-demethylation of lanosterol, transforming lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-β-ol. CYP51A1 is ubiquitously expressed, with highest levels found in liver, ovary, testis, lung, kidney and prostate.

Immunogen: E.coli-derived human CYP51A1/CYP51 recombinant protein (Position: K141-K503).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Aldahmesh, M. A., Khan, A. O., Mohamed, J. Y., Hijazi, H., Al-Owain, M., Alswaid, A., Alkuraya, F. S. Genomic analysis of pediatric cataract in Saudi Arabia reveals novel candidate disease genes. Genet. Med. 14: 955-962, 2012. 2. Gillespie, R. L., O'Sullivan, J., Ashworth, J., Bhaskar, S., Williams, S., Biswas, S., Kehdi, E., Ramsden, S. C., Clayton-Smith, J., Black, G. C., Lloyd, I. C. Personalized diagnosis and management of congenital cataract by next-generation sequencing. Ophthalmology 121: 2124-2137, 2014. 3. Hubler, Z., Allimuthu, D., Bederman, I., Elitt, M. S., Madhavan, M., Allan, K. C., Shick, H. E., Garrison, E., Karl, M. T., Factor, D. C., Nevin, Z. S., Sax, J. L. {and 12 others}: Accumulation of 8,9-unsaturated sterols drives oligodendrocyte formation and remyelination. Nature 560: 372-376, 2018.

Uniprot ID: Q16850

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 57 kDa

Calculated Molecular Weight: 23310 MW

Gene ID: 1595

Protein Name: Lanosterol 14-alpha demethylase

Gene Full Name: cytochrome P450 family 51 subfamily A member 1

Synonyms: CP51; CYP51; CYP51A1; CYPL1; CYPLI; Cytochrome P450 14DM; Cytochrome P450 51A1; Cytochrome P45014DM; Cytochrome P450LI; LDM; P450 14DM; P450L1; Sterol 14 alpha demethylase