Anti-CYP1B1 Antibody Picoband®

SKU: A00515

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CYP1B1

Specificity:

Background: Cytochrome P450 1B1 is an enzyme that in humans is encoded by the CYP1B1 gene. CYP1B1 belongs to the the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.

Immunogen: E.coli-derived human CYP1B1 recombinant protein (Position: R255-E540). Human CYP1B1 shares 82.9% amino acid (aa) sequence identity with mouse and rat CYP1B1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Entrez Gene: cytochrome P450. 2. Bailey LR, Roodi N, Dupont WD, Parl FF (1998). Association of cytochrome P450 1B1 (CYP1B1) polymorphism with steroid receptor status in breast cancer.. Cancer Res. 58 (22): 5038–41. 3. Michels-Rautenstrauss KG, Mardin CY, Zenker M et al. (2002). Primary congenital glaucoma: three case reports on novel mutations and combinations of mutations in the GLC3A (CYP1B1) gene.. J. Glaucoma 10 (4): 354–7.

Uniprot ID: Q16678

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 52 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 1545

Protein Name: Cytochrome P450 1B1

Gene Full Name: cytochrome P450 family 1 subfamily B member 1

Synonyms: CP1B; CYP1B1; CYPIB1; Cytochrome P450 1B1; GLC3A; P4501B1