Anti-CYP11B1 Antibody Picoband®

SKU: PA1699

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: CYP11B1

Specificity: No cross reactivity with other proteins.

Background: CYP11B1 (Cytochrome p450, family 11, subfamily B, polypeptide 1), also called Steroid 11-beta-hydroxylase or P450C11, is a steroid hydroxylase found in the zona fasciculata. The CYP11B1 gene functions primarily in mitochondria in the zona fasciculata of the adrenal cortex to convert 11-deoxycortisol to cortisol and 11-deoxycorticosterone to corticosterone. CYP11B1 is a member of the cytochrome P450 superfamily of enzymes. It is mapped on 8q24.3. The CYP11B1 gene contains 9 exons and spans 6.5 kb. Using RT-PCR, Kayes-Wandover and White detected CYP11B1 mRNA in human cardiac tissue samples from left and right atria, aorta, apex, intraventricular septum, and atrioventricular node, as well as whole adult and fetal heart. Ventricles did not express CYP11B1. In patients with 11-beta-hydroxylase deficiency leading to hypertension and congenital adrenal hyperplasia, Pascoe et al. identified mutations in the CYP11B1 gene.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CYP11B1.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Chua, S. C., Szabo, P., Vitek, A., Grzeschik, K.-H., John, M., White, P. C. Cloning of cDNA encoding steroid 11-beta-hydroxylase (P450C11). Proc. Nat. Acad. Sci. 84: 7193-7197, 1987. 2. Cicila, G. T., Rapp, J. P., Wang, J. M., St. Lezin, E., Ng, S. C., Kurtz, T. W. Linkage of 11-beta-hydroxylase mutations with altered steroid biosynthesis and blood pressure in the Dahl rat. Nature Genet. 3: 346-353, 1993. 3. Ezquieta, B., Luzuriaga, C. Neonatal salt-wasting and 11 beta-hydroxylase deficiency in a child carrying a homozygous deletion hybrid CYP11B2 (aldosterone synthase)-CYP11B1 (11 beta-hydroxylase). Clin. Genet. 66: 229-235, 2004.

Uniprot ID: P15538

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 58 kDa

Calculated Molecular Weight: 57573 MW

Gene ID: 1584/1585

Protein Name: Cytochrome P450 11B1, mitochondrial

Gene Full Name: cytochrome P450 family 11 subfamily B member 1

Synonyms: cytochrome P450 family 11 subfamily B member 1; CPN1; CYP11B; FHI; P450C11; CYP11B1