Anti-CXCR2 Antibody

SKU: A00455-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,IHC

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: CXCR2

Specificity:

Background: CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family (subfamily, chemokine). Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR2, which shares 52.4% amino acid (aa) sequence identity with rat CXCR2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Murphy P.M., Tiffany H.L.;Cloning of complementary DNA encoding a functional human interleukin-8 receptor.; Science 253:1280-1283(1991). 2. Cerretti D.P., Kozlosky C.J., Vanden Bos T., Nelson N., Gearing D.P., Beckmann M.P. Molecular characterization of receptors for human interleukin-8, GRO/melanoma growth-stimulatory activity and neutrophil activating peptide-2.; Mol. Immunol. 30:359-367(1993). 3. Sprenger H., Lloyd A.R., Lautens L.L., Bonner T.I., Kelvin D.J.;Structure, genomic organization, and expression of the human interleukin-8 receptor B gene.;J. Biol. Chem. 269:11065-11072(1994).

Uniprot ID: P25025

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 68 kDa

Calculated Molecular Weight: 22875 MW

Gene ID: 3579

Protein Name: C-X-C chemokine receptor type 2

Gene Full Name: C-X-C motif chemokine receptor 2

Synonyms: CD182; CDw128b; CMKAR2; CXC R2; CXCR 2; CXCR2; GRO/MGSA receptor; IL 8 receptor type 2; IL 8R B; IL8R2; IL8RA; IL8RB; IL-8RB; interleukin 8 receptor; beta