Anti-CSDE1/NRU Antibody Picoband®

SKU: A05114-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CSDE1

Specificity:

Background: Cold shock domain-containing protein E1 is a protein that in humans is encoded by the CSDE1 gene. Originally described as the product of an active transcription unit located upstream of the N-RAS gene, CSDE1 (cold shock domain-containing protein E1) is a highly conserved RNA-binding protein containing five cold-shock domains. CSDE1 is mostly localized in the cytoplasm where it interacts with distinct protein complexes involved in the post-transcriptional regulation of numerous mRNAs in a dynamic and complex manner. CSDE1 plays a role in maintaining the undifferentiated state of human embryonic stem cells (hESCs), and its loss results in accelerated neural differentiation and neurogenesis, partially via post-transcriptional regulation of fatty acid binding protein 7 (FABP7) and vimentin (VIM) mRNAs. In human melanoma, increased CSDE1 protein expression has been shown to modulate the levels of pro-oncogenic factors such as vimentin or RAC1 and tumor suppressors such as PTEN, thus promoting tumor invasion and metastasis.

Immunogen: E.coli-derived human CSDE1/NRU recombinant protein (Position: M1-Q775).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chang, T.-C., Yamashita, A., Chen, C.-Y. A., Yamashita, Y., Zhu, W., Durdan, S., Kahvejian, A., Sonenberg, N., Shyu, A.-B. UNR, a new partner of poly(A)-binding protein, plays a key role in translationally coupled mRNA turnover mediated by the c-fos major coding-region determinant. Genes Dev. 18: 2010-2023, 2004. 2. Doniger, J., DiPaolo, J. A. Coordinate N-RAS mRNA up-regulation with mutational activation in tumorigenic guinea pig cells. Nucleic Acids Res. 16: 969-980, 1988. 3. Edwards, M. C., Liegeois, N., Horecka, J., DePinho, R. A., Sprague, G. F., Jr., Tyers, M., Elledge, S. J. Human CPR (cell cycle progression restoration) genes impart a Far- phenotype on yeast cells. Genetics 147: 1063-1076, 1997.

Uniprot ID: O75534

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 89 kDa

Calculated Molecular Weight: 67074 MW

Gene ID: 7812

Protein Name: Cold shock domain-containing protein E1

Gene Full Name: cold shock domain containing E1

Synonyms: CSDE1; D1S155E; KIAA0885; N ras upstream gene protein; NRU; Protein UNR; RP5 1000E10.3; UNR