Anti-CLPX Antibody Picoband® (monoclonal, 11C11)

SKU: M00978

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IF,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Gene Name: CLPX

Specificity: No cross reactivity with other proteins.

Background: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial is an enzyme that in humans is encoded by the CLPX gene. This protein is a member of the family of AAA Proteins (AAA+ ATPase) and is to form the protein complex of Clp protease. The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.

Immunogen: E. coli-derived human CLPX recombinant protein (Position: A337-E574).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Reference: 1. Corydon, T. J., Wilsbech, M., Jespersgaard, C., Andresen, B. S., Borglum, A. D., Pedersen, S., Bolund, L., Gregersen, N., Bross, P. Human and mouse mitochondrial orthologs of bacterialClpX. Mammalian Genome 11: 899-905, 2000. 2. Kang, S. G., Dimitrova, M. N., Ortega, J., Ginsburg, A., Maurizi, M. R. Human mitochondrial ClpP is a stable heptamer that assembles into a tetradecamer in the presence of ClpX. J. Biol. Chem. 280: 35424-35432, 2005.

Uniprot ID: O76031

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 11C11

Observed Molecular Weight: 69 kDa

Calculated Molecular Weight:

Gene ID: 10845

Protein Name: ATP-dependent clpX-like chaperone, mitochondrial

Gene Full Name: caseinolytic mitochondrial matrix peptidase chaperone subunit X

Synonyms: ATP-dependent Clp protease ATP-binding subunit clpX-like; mitochondrial; CLPX