Anti-CHD1 Antibody Picoband®

SKU: A01175-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CHD1

Specificity:

Background: The Chromodomain-Helicase DNA-binding 1 is a protein that, in humans, is encoded by the CHD1 gene. The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Immunogen: E.coli-derived human CHD1 recombinant protein (Position: E71-D1250).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Delmas, V., Stokes, D. G., Perry, R. P. A mammalian DNA-binding protein that contains a chromodomain and an SNF2/SWI2-like helicase domain. Proc. Nat. Acad. Sci. 90: 2414-2418, 1993. 2. Flanagan, J. F., Mi, L.-Z., Chruszcz, M., Cymborowski, M., Clines, K. L., Kim, Y., Minor, W., Rastinejad, F., Khorasanizadeh, S. Double chromodomains cooperate to recognize the methylated histone H3 tail. Nature 438: 1181-1185, 2005. 3. Gaspar-Maia, A., Alajem, A., Polesso, F., Sridharan, R., Mason, M. J., Heidersbach, A., Ramalho-Santos, J., McManus, M. T., Plath, K., Meshorer, E., Ramalho-Santos, M. Chd1 regulates open chromatin and pluripotency of embryonic stem cells. Nature 460: 863-868, 2009.

Uniprot ID: O14646

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 270 kDa

Calculated Molecular Weight:

Gene ID: 1105

Protein Name: Chromodomain-helicase-DNA-binding protein 1

Gene Full Name: chromodomain helicase DNA binding protein 1

Synonyms: ATP dependent helicase CHD1; CHD 1; CHD1