Anti-Cenpe Antibody Picoband®

SKU: A04553-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Cenpe

Specificity:

Background: Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene. Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.

Immunogen: E.coli-derived mouse Cenpe recombinant protein (Position: Q1390-Q2474). Mouse Cenpe shares 49.9% amino acid (aa) sequence identity with human Cenpe, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Abrieu, A., Kahana, J. A., Wood, K. W., Cleveland, D. W. CENP-E as an essential component of the mitotic checkpoint in vitro. Cell 102: 817-826, 2000. 2. Barisic, M., Silva e Sousa, R., Tripathy, S. K., Magiera, M. M., Zaytsev, A. V., Pereira, A. L., Janke, C., Grishchuk, E. L., Maiato, H. Microtubule detyrosination guides chromosomes during mitosis. Science 348: 799-803, 2015. 3. Gross, M. B. Personal Communication. Baltimore, Md. 11/4/2014.

Uniprot ID: Q6RT24

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 300 kDa

Calculated Molecular Weight:

Gene ID: 229841

Protein Name: Centromere-associated protein E

Gene Full Name: centromere protein E

Synonyms: Cenpe; Centromere-associated protein E; Centromere protein E; CENP-E; Kinesin superfamily protein 10; KIF10; Motor domain of KIF10