Anti-CDK12 Antibody Picoband®

SKU: A03495-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IP,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry(Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: CDK12

Specificity:

Background: CDK12 cyclin-dependent kinase 12 is a protein kinase that in humans is encoded by the CDK12 gene. This enzyme is a member of cyclin-dependent kinase protein family. CDK12 forms protein complexes with cyclin K (CCNK). The CDK12-CCNK complex acts at the level of RNA polymerase II (RNAPII) to regulate expression of long complex genes, several of which have roles in DNA maintenance and repair。

Immunogen: E.coli-derived human CDK12 recombinant protein (Position: Q1147-N1439).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Reference: 1. Blazek, D., Kohoutek, J., Bartholomeeusen, K., Johansen, E., Hulinkova, P., Luo, Z., Cimermancic, P., Ule, J., Peterlin, B. M. The cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes. Genes Dev. 25: 2158-2172, 2011. 2. Cheng, S.-W. G., Kuzyk, M. A., Moradian, A., Ichu, T.-A., Chang, V. C.-D., Tien, J. F., Vollett, S. E., Griffith, M., Marra, M. A., Morin, G. B. Interaction of cyclin-dependent kinase 12/CrkRS with cyclin K1 is required for the phosphorylation of the C-terminal domain of RNA polymerase II. Molec. Cell. Biol. 32: 4691-4704, 2012. 3. Dubbury, S. J., Boutz, P. L., Sharp, P. A. CDK12 regulates DNA repair genes by suppressing intronic polyadenylation. Nature 564: 141-145, 2018.

Uniprot ID: Q9NYV4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 205 kDa

Calculated Molecular Weight: 79995 MW

Gene ID: 51755

Protein Name: Cyclin-dependent kinase 12

Gene Full Name: cyclin dependent kinase 12

Synonyms: CDC2 related protein kinase 7, CDK12, CDK12/CRKRS, CRK7, CRKR, CRKRS, KIAA0904