Anti-CD3D Antibody Picoband®

SKU: A04405-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.2-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CD3D

Specificity:

Background: T-cell surface glycoprotein CD3 delta chain is a protein that in humans is encoded by the CD3D gene. The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.

Immunogen: E.coli-derived human CD3D recombinant protein (Position: F22-K171). Human CD3D shares 66% and 70.7% amino acid (aa) sequence identity with mouse and rat CD3D, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dadi, H. K., Simon, A. J., Roifman, C. M. Effect of CD3-delta deficiency on maturation of alpha/beta and gamma/delta T-cell lineages in severe combined immunodeficiency. New Eng. J. Med. 349: 1821-1828, 2003. Note: Erratum: New Eng. J. Med. 350: 1803 only, 2004. 2. de Saint Basile, G., Geissmann, F., Flori, E., Uring-Lambert, B., Soudais, C., Cavazzana-Calvo, M., Durandy, A., Jabado, N., Fischer, A., Le Diest, F. Severe combined immunodeficiency caused by deficiency in either the delta or the epsilon subunit of CD3. J. Clin. Invest. 114: 1512-1517, 2004. 3. Delgado, P., Fernandez, E., Dave, V., Kappes, D., Alarcon, B. CD3-delta couples T-cell receptor signalling to ERK activation and thymocyte positive selection. Nature 406: 426-430, 2000.

Uniprot ID: P04234

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 19 kDa

Calculated Molecular Weight:

Gene ID: 915

Protein Name: T-cell surface glycoprotein CD3 delta chain

Gene Full Name: CD3 delta subunit of T-cell receptor complex

Synonyms: CD3 delta; CD3D; CD3-delta; T cell receptor T3 delta chain; T3D; TCRδ