Anti-CD13/ANPEP Picoband® Antibody (monoclonal, 5B9)

SKU: M02591-4

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat,Monkey

Applications: WB,IHC

Application Details: Western blot, 0.25-0.5μg/ml, Human, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat

Gene Name: ANPEP

Specificity:

Background: Membrane alanyl aminopeptidase (EC 3.4.11.2) also known as alanyl aminopeptidase (AAP) or aminopeptidase N (AP-N) is an enzyme that in humans is encoded by the ANPEP gene. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.

Immunogen: E.coli-derived human CD13/ANPEP recombinant protein (Position: D148-S966).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Delmas, B., Gelfi, J., L'Haridon, R., Vogel, L. K., Sjostrom, H., Noren, O., Laude, H. Aminopeptidase N is a major receptor for the enteropathogenic coronavirus TGEV. Nature 357: 417-420, 1992. 2. Kruse, T. A., Bolund, L., Grzeschik, K.-H., Ropers, H. H., Olsen, J., Sjostrom, H., Noren, O. Assignment of the human aminopeptidase N (peptidase E) gene to chromosome 15q13-qter. FEBS Lett. 239: 305-308, 1988. 3. Look, A. T., Ashmun, R. A., Shapiro, L. H., Peiper, S. C. Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N. J. Clin. Invest. 83: 1299-1307, 1989.

Uniprot ID: P15144

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 5B9

Observed Molecular Weight: 150 kDa

Calculated Molecular Weight:

Gene ID: 290

Protein Name: Aminopeptidase N

Gene Full Name: alanyl aminopeptidase, membrane

Synonyms: Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; AP M; AP N; APN; CD13; gp150; hAPN; LAP1; Microsomal aminopeptidase; p150; PEPN