Anti-CAPN5 Antibody Picoband®

SKU: A08350-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CAPN5

Specificity:

Background: Calpain-5 is a protein that in humans is encoded by the CAPN5 gene. Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.

Immunogen: E.coli-derived human CAPN5 recombinant protein (Position: N583-N616). Human CAPN5 shares 84.8% and 78.8% amino acid (aa) sequence identity with mouse and rat CAPN5, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bennett, S. R., Folk, J. C., Kimura, A. E., Russell, S. R., Stone, E. M., Raphtis, E. M. Autosomal dominant neovascular inflammatory vitreoretinopathy. Ophthalmology 97: 1125-1135, 1990. 2. Dear, N., Matena, K., Vingron, M., Boehm, T. A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution. Genomics 45: 175-184, 1997. 3. Franz, T., Winckler, L., Boehm, T., Dear, T. N. Capn5 is expressed in a subset of T cells and is dispensable for development. Molec. Cell. Biol. 24: 1649-1654, 2004.

Uniprot ID: O15484

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 73 kDa

Calculated Molecular Weight:

Gene ID: 726

Protein Name: Calpain-5

Gene Full Name: calpain 5

Synonyms: CAPN5; NCL3; Calpain-5; Calpain htra-3; New calpain 3; nCL-3