Anti-C14orf166/RTRAF Antibody Picoband®

SKU: A31823-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC/IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: RTRAF

Specificity:

Background: Enables RNA binding activity; RNA polymerase II complex binding activity; and identical protein binding activity. Involved in negative regulation of protein kinase activity; positive regulation of transcription by RNA polymerase II; and tRNA splicing, via endonucleolytic cleavage and ligation. Located in microtubule cytoskeleton; nucleoplasm; and perinuclear region of cytoplasm. Part of tRNA-splicing ligase complex.

Immunogen: E.coli-derived human C14orf166/RTRAF recombinant protein (Position: M1-R244). Human C14orf166/RTRAF shares 97.1% amino acid (aa) sequence identity with mouse C14orf166/RTRAF.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 12/18/2014. 2. Howng, S.-L., Hsu, H.-C., Cheng, T.-S., Lee, Y.-L., Chang, L.-K., Lu, P.-J., Hong, Y.-R. A novel ninein-interaction protein, CGI-99, blocks ninein phosphorylation by GSK3-beta and is highly expressed in brain tumors. FEBS Lett. 566: 162-168, 2004. 3. Huarte, M., Sanz-Ezquerro, J. J., Roncal, F., Ortin, J., Nieto, A. PA subunit from influenza virus polymerase complex interacts with a cellular protein with homology to a family of transcriptional activators. J. Virol. 75: 8597-8604, 2001.

Uniprot ID: Q9Y224

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 28 kDa

Calculated Molecular Weight: 28 kDa

Gene ID: 51637

Protein Name: RNA transcription, translation and transport factor protein

Gene Full Name: RNA transcription, translation and transport factor

Synonyms: RTRAF; CLE7 homolog; CLE7; CLE; CGI-99