Anti-BRSK2 Antibody Picoband®

SKU: A05321-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: BRSK2

Specificity:

Background: BR serine/threonine-protein kinase 2 is an enzyme that in humans is encoded by the BRSK2 gene. BRSK2 was initially identified through a computer screen of the human genome and shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK2 is expressed in the brain and to a lesser extent in the testes. BRSK2 is a member of the AMP-activated protein kinase subfamily and can be activated by the tumor suppressor kinase LKB1. More recently, it has been shown that both BRSK2 and the related protein BRSK1 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. BRSK2 has also been shown to be an autoantigen in paraneoplastic limbic encephalitis. At least four isoforms of BRSK2 are known to exist.

Immunogen: E.coli-derived human BRSK2 recombinant protein (Position: M250-P736).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kishi, M., Pan, Y. A., Crump, J. G., Sanes, Y. R. Mammalian SAD kinases are required for neuronal polarization. Science 307: 929-932, 2005. 2. Lu, R., Niida, H., Nakanishi, M. Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function. J. Biol. Chem. 279: 31164-31170, 2004. 3. Miura, K., Masuzaki, H., Ishimaru, T., Niikawa, N., Jinno, Y. A HhaI/BstUI polymorphism in a novel gene at human chromosome 11p15.5. J. Hum. Genet. 43: 283-284, 1998.

Uniprot ID: Q8IWQ3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 90 kDa

Calculated Molecular Weight: 39411 MW

Gene ID: 9024

Protein Name: Serine/threonine-protein kinase BRSK2

Gene Full Name: BR serine/threonine kinase 2

Synonyms: BR serine/threonine kinase 2; BRSK2; C11orf7; PEN11B; SAD1; STK29