Anti-BCAT1 Antibody Picoband®

SKU: PA1538

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: BCAT1

Specificity: No cross reactivity with other proteins.

Background: BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT1, different from the related mouse sequence by one amino acid, rat sequence by three amino acids.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Naylor, S. L., Shows, T. B.Branched-chain aminotransferase genes (BCT-1 and BCT-2) assigned to human chromosomes 12 and 19 using alpha-keto acid selection media. (Abstract)Cytogenet. Cell Genet. 25: 191-192, 1979. 2. Jones, C., Moore, E. E.Isolation of mutants lacking branched-chain amino acid transaminase.Somat. Cell Genet. 2: 235-243, 1976.

Uniprot ID: P54687

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 43 kDa

Calculated Molecular Weight: 43 kDa

Gene ID: 586

Protein Name: Branched-chain-amino-acid aminotransferase, cytosolic

Gene Full Name: branched chain amino acid transaminase 1

Synonyms: BCAT1; BCAT1/ECA39; ECA39; MECA39; PNAS 121; PP18; Protein ECA39