Anti-B Raf/BRAF Antibody Picoband®

SKU: PA1848

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: BRAF

Specificity: No cross reactivity with other proteins.

Background: BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf, also known as ONCOGENE BRAF, BRAF1, RAFB1, Proto-oncogene B-Raf p94, v-Raf murine sarcoma viral oncogene homolog B1, proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1, while the protein is more formally known as serine/threonine-protein kinase B-Raf. BRAF deduced 651-amino acid protein has a calculated molecular mass of 72.5 kD. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts.B-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human B Raf, identical to the related rat and mouse sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Eychene, A., Barnier, J. V., Apiou, F., Dutrillaux, B., Calothy, G. Chromosomal assignment of two human B-raf(Rmil) proto-oncogene loci: B-raf-1 encoding the p94(Braf/Rmil) and B-raf-2, a processed pseudogene.Oncogene 7: 1657-1660, 1992. 2. Hishinuma, A., Fukata, S., Kakudo, K., Murata, Y., Ieiri, T. High incidence of thyroid cancer in long-standing goiters with thyroglobulin mutations. Thyroid 15: 1079-1084, 2005. 3. Sithanandam, G., Kolch. W., Duh, F.-M., Rapp, U. R.Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies.Oncogene 5: 1775-1780, 1990.

Uniprot ID: P15056

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 84 kDa

Calculated Molecular Weight: 84 kDa

Gene ID: 673

Protein Name: Serine/threonine-protein kinase B-raf

Gene Full Name: B-Raf proto-oncogene, serine/threonine kinase

Synonyms: B RAF1; BRAF; BRAF1; p94; Proto oncogene B Raf; RAFB1