Anti-ATP4B Antibody Picoband®

SKU: A08719-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunofluorescence, 5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ATP4B

Specificity:

Background: This gene encodes the beta subunit of the gastric H+, K+-ATPase. The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion.

Immunogen: E.coli-derived human ATP4B recombinant protein (Position: Y60-D277). Human ATP4B shares 82.8% and 80.5% amino acid (aa) sequence identity with mouse and rat ATP4B, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Canfield, V. A., Okamoto, C. T., Chow, D., Dorfman, J., Gros, P., Forte, J. G., Levenson, R. Cloning of the H,K-ATPase beta subunit: tissue-specific expression, chromosomal assignment, and relationship to Na,K-ATPase beta subunits. J. Biol. Chem. 265: 19878-19884, 1990. 2. Reuben, M. A., Lasater, L. S., Sachs, G. Characterization of a beta subunit of the gastric H(+)/K(+)-transporting ATPase. Proc. Nat. Acad. Sci. 87: 6767-6771, 1990. 3. Song, I., Brown, D. R., Yamada, T., Trent, J. M. Mapping of the gene encoding the beta-subunit of H(+), K(+)-ATPase to human chromosome 13q34 by fluorescence in situ hybridization. Genomics 14: 1114-1115, 1992.

Uniprot ID: P51164

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 75 kDa

Calculated Molecular Weight: 33 kDa

Gene ID: 496

Protein Name: Potassium-transporting ATPase subunit beta

Gene Full Name: ATPase H+/K+ transporting subunit beta

Synonyms: ATP4B; ATP6B; H+/K+ ATPase beta; Proton pump beta chain