Anti-Arp2/ACTR2 Antibody Picoband®

SKU: A03898-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Monkey,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 4μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -

Gene Name: ACTR2

Specificity:

Background: The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human Arp2/ACTR2 recombinant protein (Position: M1-R394).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Caridi, C. P., D'Agostino, C., Ryu, T., Zapotoczny, G., Delabaere, L., Li, X., Khodaverdian, V. Y., Amaral, N., Lin, E., Rau, A. R., Chiolo, I. Nuclear F-actin and myosins drive relocalization of heterochromatic breaks. Nature 559: 54-60, 2018. 2. Hanisch, J., Kolm, R., Wozniczka, M., Bumann, D., Rottner, K., Stradal, T. E. B. Activation of a RhoA/myosin II-dependent but Arp2/3 complex-independent pathway facilitates Salmonella invasion. Cell Host Microbe 9: 273-285, 2011. 3. Li, P., Banjade, S., Cheng, H.-C., Kim, S., Chen, B., Guo, L., Llaguno, M., Hollingsworth, J. V., King, D. S., Banani, S. F., Russo, P. S., Jiang, Q.-X., Nixon, B. T., Rosen, M. K. Phase transitions in the assembly of multivalent signalling proteins. Nature 483: 336-340, 2012.

Uniprot ID: P61160

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 45 kDa

Calculated Molecular Weight:

Gene ID: 10097

Protein Name: Actin-related protein 2

Gene Full Name: actin related protein 2

Synonyms: Actin like protein 2; Actin related protein 2; ACTR2; ARP2