Anti-ARL1 Antibody Picoband®

SKU: A03733-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Human

Gene Name: ARL1

Specificity:

Background: ADP-ribosylation factor-like protein 1 is a protein that in humans is encoded by the ARL1 gene. The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARL1, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 7/25/2012. 2. Hong, J.-X., Lee, F.-J. S., Patton, W. A., Lin, C. Y., Moss, J., Vaughan, M. Phospholipid- and GTP-dependent activation of cholera toxin and phospholipase D by human ADP-ribosylation factor-like protein 1 (HARL1). J. Biol. Chem. 273: 15872-15876, 1998. 3. Lu, L., Tai, G., Hong, W. Autoantigen golgin-97, an effector of Arl1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Molec. Biol. Cell 15: 4426-4443, 2004.

Uniprot ID: P40616

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 20 kDa

Calculated Molecular Weight: 26934 MW

Gene ID: 400

Protein Name: ADP-ribosylation factor-like protein 1

Gene Full Name: ARF like GTPase 1

Synonyms: ADP ribosylation factor like 1; ARFL1; ARL1