Anti-ARHGEF5 Antibody Picoband®

SKU: A01487-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ARHGEF5

Specificity:

Background: Rho guanine nucleotide exchange factor 5 is a protein that in humans is encoded by the ARHGEF5 gene. Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.

Immunogen: E.coli-derived human ARHGEF5 recombinant protein (Position: D1555-A1597). Human ARHGEF5 shares 97.6% amino acid (aa) sequence identity with mouse ARHGEF5.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chan, A. M-L., McGovern, E. S., Catalano, G., Fleming, T. P., Miki, T. Expression cDNA cloning of a novel oncogene with sequence similarity to regulators of small GTP-binding proteins. Oncogene 9: 1057-1063, 1994. 2. Debily, M.-A., Camarca, A., Ciullo, M., Mayer, C., El Marhomy, S., Ba, I., Jalil, A., Anzisi, A., Guardiola, J., Piatier-Tonneau, D. Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer. Hum. Molec. Genet. 13: 323-334, 2004. 3. Miki, T., Fleming, T. P., Crescenzi, M., Molloy, C. J., Blam, S. B., Reynolds, S. H., Aaronson, S. A. Development of a highly efficient expression cDNA cloning system: application to oncogene isolation. Proc. Nat. Acad. Sci. 88: 5167-5171, 1991.

Uniprot ID: Q12774

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 250 kDa

Calculated Molecular Weight:

Gene ID: 7984

Protein Name: Rho guanine nucleotide exchange factor 5

Gene Full Name: Rho guanine nucleotide exchange factor 5

Synonyms: ARHGEF5; TIM; Rho guanine nucleotide exchange factor 5; Ephexin-3; Guanine nucleotide regulatory protein TIM; Oncogene TIM; Transforming immortalized mammary oncogene; p60 TIM