Anti-APE1 APEX1 Antibody Picoband® (monoclonal, 5C11)

SKU: M00627

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,IHC,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Gene Name: APEX1

Specificity: No cross reactivity with other proteins.

Background: APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.

Immunogen: E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat APE1, respectively.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Akiyama, K., Seki, S., Oshida, T., Yoshida, M. C.Structure, promoter analysis and chromosomal assignment of the human APEX gene.Biochim. Biophys. Acta 1219: 15-25, 1994. 2. Chou, K.-M., Cheng, Y.-C.An exonucleolytic activity of human apurinic/apyrimidinic endonuclease on 3-prime mispaired DNA.Nature 415: 655-659, 2002. 3. Robson, C. N., Hochhauser, D., Craig, R., Rack, K., Buckle, V. J., Hickson, I. D.Structure of the human DNA repair gene HAP1 and its localisation to chromosome 14q11.2-12.Nucleic Acids Res. 20: 4417-4421, 1992.

Uniprot ID: P27695

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 5C11

Observed Molecular Weight: 39 kDa

Calculated Molecular Weight:

Gene ID: 328

Protein Name: DNA repair nuclease/redox regulator APEX1

Gene Full Name: apurinic/apyrimidinic endodeoxyribonuclease 1

Synonyms: AP endonuclease 1; APE; APE1; APE1; APEX1; APEN; APEX; APEX nuclease; APEX1; APX; HAP1; Protein REF 1; REF1