Anti-Angiotensin Converting Enzyme 1/Ace Antibody Picoband®

SKU: A00251

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: Flow Cytometry,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Mouse

Gene Name: Ace

Specificity: No cross reactivity with other proteins.

Background: Angiotensin I converting enzyme (ACE), also called DCP or CD143 is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. This gene is mapped to 17q23.3. This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Ace, which shares 70.3% and 91.9% amino acid (aa) sequence identity with human and rat Ace, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Catarsi, P., Ravazzolo, R., Emma, F., Fruci, D., Finos, L., Frau, A., Morreale, G., Carrea, A., Ghiggeri, G. M. Angiotensin-converting enzyme (ACE) haplotypes and cyclosporine A (CsA) response: a model of the complex relationship between ACE quantitative trait locus and pathological phenotypes. Hum. Molec. Genet. 14: 2357-2367, 2005. 2. Hamdi, H. K., Reznik, J., Castellon, R., Atilano, S. R., Ong, J. M., Udar, N., Tavis, J. H., Aoki, A. M., Nesburn, A. B., Boyer, D. S., Small, K. W., Brown, D. J., Kenney, M. C. Alu DNA polymorphism in ACE gene is protective for age-related macular degeneration. Biochem. Biophys. Res. Commun. 295: 668-672, 2002.

Uniprot ID: P09470

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 150-180 kDa

Calculated Molecular Weight: 54 kDa

Gene ID: 11421

Protein Name: Angiotensin-converting enzyme

Gene Full Name: angiotensin I converting enzyme

Synonyms: ACE; ACE1; Angiotensin converting enzyme; CD143; DCP; DCP1; Dipeptidyl carboxypeptidase I; Kininase II; MVCD3