Anti-ACOX1 Antibody Picoband®

SKU: A03054-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC/IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: ACOX1

Specificity:

Background: The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified.

Immunogen: E.coli-derived human ACOX1 recombinant protein (Position: A605-E648). Human ACOX1shares 93.2% and 90.9% amino acid (aa) sequence identity with mouse and rat ACOX1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aoyama, T., Tsushima, K., Souri, M., Kamijo, T., Suzuki, Y., Shimozawa, N., Orii, T., Hashimoto, T. Molecular cloning and functional expression of a human peroxisomal acyl-coenzyme A oxidase. Biochem. Biophys. Res. Commun. 198: 1113-1118, 1994. 2. Carrozzo, R., Bellini, C., Lucioli, S., Deodato, F., Cassandrini, D., Cassanello, M., Caruso, U., Rizzo, C., Rizza, T., Napolitano, M. L., Wanders, R. J. A., Jakobs, C., Bruno, C., Santorelli, F. M., Dionisi-Vici, C., Bonioli, E. Peroxisomal acyl-CoA-oxidase deficiency: two new cases. Am. J. Med. Genet. 146A: 1676-1681, 2008. 3. Chung, H., Wangler, M. F., Marcogliese, P. C., Jo, J., Ravenscroft, T. A., Zuo, Z., Duraine, L., Sadeghzadeh, S., Li-Kroeger, D., Schmidt, R. E., Pestronk, A., Rosenfeld, J. A., and 23 others. Loss- or gain-of-function mutations in ACOX1 cause axonal loss via different mechanisms. Neuron 106: 589-606, 2020.

Uniprot ID: Q15067

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 22, 74 kDa

Calculated Molecular Weight: 74 kDa

Gene ID: 51

Protein Name: Peroxisomal acyl-coenzyme A oxidase 1

Gene Full Name: acyl-CoA oxidase 1

Synonyms: Peroxisomal acyl-CoA oxidase 1; A chain; Palmitoyl-CoA oxidase; EC:1.3.3.6; AOX; ACOX 1