Anti-ABL1 Antibody Picoband®

SKU: A00133-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ABL1

Specificity:

Background: c Abl is also called as ABL1. This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene. Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons.

Immunogen: E.coli-derived human ABL1 recombinant protein (Position: L1012-Q1049). Human ABL1 shares 97.4% amino acid (aa) sequence identity with mouse ABL1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Entrez Gene: ABI1 abl-interactor 1. 2. Biesova Z, Piccoli C, Wong WT (Feb 1997). Isolation and characterization of e3B1, an eps8 binding protein that regulates cell growth. Oncogene 14 (2): 233–41. 3. Ziemnicka-Kotula D, Xu J, Gu H, Potempska A, Kim KS, Jenkins EC, Trenkner E, Kotula L (Jul 1998). Identification of a candidate human spectrin Src homology 3 domain-binding protein suggests a general mechanism of association of tyrosine kinases with the spectrin-based membrane skeleton. J Biol Chem 273 (22): 13681–92.

Uniprot ID: P00519

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 143 kDa

Calculated Molecular Weight:

Gene ID: 25

Protein Name: Tyrosine-protein kinase ABL1

Gene Full Name: ABL proto-oncogene 1, non-receptor tyrosine kinase

Synonyms: ABL1; ABL; JTK7; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150