Anti-AATF Antibody Picoband®

SKU: A03945-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: AATF

Specificity:

Background: Protein AATF, also known as apoptosis-antagonizing transcription factor is a protein that in humans is encoded by the AATF gene. The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

Immunogen: E.coli-derived human AATF recombinant protein (Position: Q5-H552). Human AATF shares 78.5% and 79.9% amino acid (aa) sequence identity with mouse and rat AATF, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Di Padova, M., Bruno, T., De Nicola, F., Iezzi, S., D'Angelo, C., Gallo, R., Nicosia, D., Corbi, N., Biroccio, A., Floridi, A., Passananti, C., Fanciulli, M. Che-1 arrests human colon carcinoma cell proliferation by displacing HDAC1 from the p21(WAF1/CIP1) promoter. J. Biol. Chem. 278: 36496-36504, 2003. 2. Fanciulli, M., Bruno, T., Di Padova, M., De Angelis, R., Iezzi, S., Iacobini, C., Floridi, A., Passananti, C. Identification of a novel partner of RNA polymerase II subunit 11, Che-1, which interacts with and affects the growth suppression function of Rb. FASEB J. 14: 904-912, 2000. 3. Lindfors, K., Halttunen, T., Huotari, P., Nupponen, N., Vihinen, M., Visakorpi, T., Maki, M., Kainulainen, H. Identification of novel transcription factor-like gene from human intestinal cells. Biochem. Biophys. Res. Commun. 276: 660-666, 2000.

Uniprot ID: Q9NY61

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 95 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 26574

Protein Name: Protein AATF

Gene Full Name: apoptosis antagonizing transcription factor

Synonyms: AATF; CHE1; DED; HSPC277; Protein AATF; Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1