AF/LE Purified Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]

SKU: GBS-F10940

Size:1mg

Storage:Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Flow Cytometry Antibodies

Abbreviation:CD90.2/Thy1.2

Target Synonym:CD90.2;Thy-1.2;Thy-1.2 antigen;Thy-1.2 membrane glycoprotein;Thy1.2

Research Areas:Immunology

Conjugation:None (AF/LE)

Host:Rat

Species reactivity:Mouse

Application:FCM

Usage:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype:Rat IgG2b, κ

Clonality:Monoclonal

Clone NO.:30H12

UNIProt ID:P01831

Background:CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Concentration:≥ 1 mg/mL

Control catalog number:

Immunogen:Recombinant Mouse CD90.2 protein

Buffer:Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Purification method:>98%, Protein A/G purified

Dilution:FCM 2 μg/mL(0.5×10⁶-1×10⁶ cells)

GeneID:21838