AF/LE Purified Anti-Mouse CD161/NK1.1 Antibody[PK136]

SKU: GBS-F09870

Size:1mg

Storage:Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Flow Cytometry Antibodies

Abbreviation:NK1.1

Target Synonym:CD161 antigen-like family member C;CD161/NK1.1;CD161c;Killer cell lectin-like receptor subfamily B member 1C;Klrb1c;Ly-55c;NKR-P1 40;NKR-P1.9;NKR-P1C

Research Areas:Immunology;Innate Immunity

Conjugation:None (AF/LE)

Host:Mouse

Species reactivity:Mouse

Application:FCM

Usage:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype:Mouse IgG2a, κ

Clonality:Monoclonal

Clone NO.:PK136

UNIProt ID:P27814;P27812;Q99JB4;

Background:NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Concentration: ≥ 1 mg/mL

Control catalog number:

Immunogen:Recombinant Mouse NK1.1 protein

Buffer:Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Purification method:>98%, Protein A/G purified

Dilution:FCM 2 μg/mL(0.5×10⁶-1×10⁶ cells)

GeneID:17059;80782