ACTb(Actin Beta) ELISA Kit

SKU: ELK8833

Size:96T

Reactivity:General

Alternative Names:ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed

Assay Type:Competitive Inhibition

Sensitivity:0.057 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Signal transduction;Developmental science;Bone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ACTb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ACTb in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: