2-AG(2-Arachidonoylglycerol) ELISA Kit

SKU: ELK8163

Size:96T

Reactivity:General

Alternative Names:2-AG

Assay Type:Competitive Inhibition

Sensitivity:1.41 ng/mL

Standard:300 ng/mL

Detection range:4.69-300 ng/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Neuro science

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 2-AG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 2-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 2-AG in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: