1,5-AG(1,5-Anhydroglucitol) ELISA Kit

SKU: ELK7840

Size:96T

Reactivity:General

Alternative Names:LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol

Assay Type:Competitive Inhibition

Sensitivity:0.6 µg/mL

Standard:150 µg/mL

Detection range:2.35-150 µg/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve.

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