Fast Mutagenesis System

The method works  first by having primers anneal to the DNA template.  Mutant strands are then synthesized with 2×TransStart® FastPfu PCR SuperMix with high fidelity. In the next step, non-mutated parental plasmid (methylated plasmid) is digested in vitro with DMT enzyme and in vivo further digested within DMT Chemically Competent Cell. 

More details

CAD$187.00

  • 10 reactions
  • 20 reactions

More info

Highlights

• Mutation sites on both primers to improve mutation efficiency.

• Partially overlapping primers for exponential DNA amplification.

• Fast (4 kb/min) and high fidelity ( 54-fold fidelity as compared to EasyTaq® DNA Polymerase) 2xTransStart® FastPfu PCR SuperMix for DNA amplification.

• Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency.

Storage

Competent Cell at -70°C for six months; others at -20°C for two years

Kit Content

Component

FM111-01 (10 rxns)

FM111-02 (20 rxns)

TransStart® FastPfu PCR SuperMix

250 µl

500 µl

DMT Enzyme (10 units/μl)

10 µl

20 µl

DMT Chemically Competent Cell

10×50 µl

20×50 µl

ddH2O

1 ml

1 ml

SControl Plasmid (5 ng/µl)

10 µl

20 µl

SControl Primers (10 µM )

10 µl

20 µl

Papers citing this product: 

LiteratureJournalIFAuthorDateLink
Whole-genome sequencing of the snub-nosed monkey provides insights into folivory and evolutionary history.Nature genetics29.648Xuming Zhou, et al.2014 DecThe original link
Crystal structure of PXY-TDIF complex reveals a conserved recognition mechanism among CLE peptide-receptor pairsCell Research14.812Heqiao Zhang, et al. Ministry of Education Key Lab2016 janThe original link
Maternal xNorrin, a canonical Wnt signaling agonist and TGF-β antagonist, controls early neuroectoderm specification in XenopusPloS Biology12.69Xu S, et al. State Key Laboratory of Molecular Dev2012The original link
PGE2 Promotes Hepatic Biliary Cholesterol Excretion by EP3-mediated HNF4α/CYP7A1 Pathway in Micehepatology11.711Shuai Yan,et al.2016DecThe original link
Plastid-nucleus communication involves calcium-modulated MAPK signallingNature communications11.329Hailong Guo, et al. Photosynthesis Research Center2016 junThe original link

1 other product in the same category:

Download

Fast mutagen sys

Fast Mutagenesis System

Download (1.03M)