Equalbit 1x dsDNA HS Assay Kit

EQ121-01

Product Overview: The Equalbit 1 × dsDNA HS Assay Kit is a highly sensitive and accurate fluorescence-based detection kit designed for the quantification of double-stranded DNA (dsDNA). This kit includes a pre-mixed working solution with fluorescent dye and dsDNA standards, ensuring a simple and efficient workflow. It offers excellent linearity within the range of 0.2 - 100 ng dsDNA, allowing for precise quantification of dsDNA concentrations from 10 pg/μl to 100 ng/μl. The kit is also highly tolerant to common contaminants such as RNA, salts, free nucleotides, proteins, solvents, and detergents, making it a robust choice for various applications.

Key Features:

• Simple Operation: The ready-to-use pre-mixed working solution simplifies the assay process by eliminating the need for additional preparation steps.
• High Sensitivity and Precision: Accurately quantifies dsDNA in the range of 0.2 - 100 ng, with a detection limit as low as 10 pg/μl.
• High Specificity: Specifically detects dsDNA while tolerating common contaminants, ensuring reliable results.
• Rapid Dye Binding: Achieves saturation within 2 minutes, allowing for quick and efficient assays.
• High Stability: The production process of standards is strictly controlled to ensure lot-to-lot consistency and stability.

 

Components:

• Equalbit 1 × dsDNA HS Working Solution
• Equalbit 1 × dsDNA HS Standard #1 (0 ng/μl in TE buffer)
• Equalbit 1 × dsDNA HS Standard #2 (10 ng/μl in TE buffer)

Storage Instructions:

• Store the kit at 2 ~ 8°C and protect it from light.
• For long-term storage, store Equalbit 1 × dsDNA HS Standard #2 at -30 ~ -15°C.
• Adjust the shipping method according to different destinations to maintain product integrity.

Applications:

• Suitable for the detection of dsDNA concentrations ranging from 10 pg/μl to 100 ng/μl.
• Ideal for use with the Qubit Fluorometer for simple and accurate dsDNA quantification.

Notes:

• To avoid contamination, transfer a sufficient amount of the working solution to a centrifuge tube before use.
• Mix standards and samples thoroughly by inversion before use to ensure accurate results.
• Use a calibrated pipette to ensure the accuracy of quantitative results.
• Perform the quantitative assay at room temperature and avoid prolonged exposure to light during the experiment.
• Complete the detection within 3 hours of adding samples to prevent fluorescence quenching.

Experiment Process:

1. Equilibrate all components to room temperature before use.
2. Prepare 0.5 ml PCR tubes for all samples and standards.
3. Label the lid of each standard and sample tube correctly.
4. Prepare the standard solution by adding 190 µl of the working solution to standard PCR tubes, then add 10 µl of Standard #1 and Standard #2 to the corresponding tubes. Gently vortex to avoid bubbles.
5. Prepare the sample solution by adding 180 - 199 µl of the working solution to sample PCR tubes, then add 1 - 20 µl of dsDNA samples to achieve a final volume of 200 µl. Gently vortex to avoid bubbles.
6. Incubate all PCR tubes at room temperature for 2 minutes, protecting them from light.
7. Follow the Qubit Fluorometer instructions to assay the dsDNA concentration using the dsDNA High Sensitivity Assay program.