pEASY®-T3 Cloning Kit

pEASY ®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released with a single enzyme digestion.

•5 minutes fast ligation

•Ampicillin resistance gene marker

•Blue/white selection

•T7, SP6 promoters, M13 forward and M13 reverse primers for sequencing.

•Includes competent cells

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CAD$296.00

  • 20 reactions
  • 60 reactions

More info

Description

pEASY ®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released from a single enzyme digestion.

•5 minutes fast ligation of Taq-amplified PCR products.

•Ampicillin resistance genes for selection.

•Easy blue/white selection.

•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.

•T7 promoter and SP6 promoter for in vitro transcription.

•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

Competent Cell at -70°C for six months; others at -20°C for nine months

Kit Content

Component

CT301-01

CT301-02

(20 rxns)

(60 rxns)

pEASY ®-T3 Cloning Vector (10 ng/μl)

20µl

3×20µl

Control Template (5 ng/μl)

5µl

5µl

Control Primers (10 μM)

5µl

5µl

M13 Forward Primer (10 μM)

50µl

150µl

M13 Reverse Primer (10 μM)

50µl

150µl

SR Primer (10 μM)

10×100µl

30×100µl

Trans1-T1 Phage Resistant Chemically Competent Cell

References:

LiteratureJournalIFAuthorDateLink
Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching.Nature Biotechnology39.082013 SepThe original link
Genome-wide screening and functional analysis identify a large number of long noncoding RNAs involved in the sexual reproduction of riceGenome Biology10.465Yu-Chan Zhang, et al.2014 DecThe original link
Prevalence of antibiotic resistance genes in soils after continually applied with different animal manure for 30 yearsjournal of hazardous materials6.065Shuang Peng,et al.2017QctThe original link
Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coliScientific Reports5.578Jie Gao, et al.2016 JanThe original link
Identification of ethylene-responsive genes in ethrel-treated shoot apices of cucumber by suppression subtractive hybridizationPlant Molecular Biology Reporter5.319Jin X, et al. Department of Horticulture, Northeas2011 DecThe original link

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