Alkaline phosphatase (AP) is highly expressed in pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). In the iPSCs induction process, the activation of AP is also necessary for successful reprogramming. In traditional AP detection methods, cells are fixed and cannot be used for further studies.
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This kit provides a fast, simple and sensitive method for AP detection without fixing the cells. The reagent is nontoxic to the cells, and the fluorescent signal will be discharged from the cells by exocytosis completely within two hours after removal of the staining solution, allowing the cell being used for following studies.
• Do not use pluripotent stem cell culture medium to dilute the 500×AP Live Solution. Do not observe the result in pluripotent stem
cell culture medium.
• Since AP is ubiquitously expressed in most cell types and highest in pluripotent stem cells. It is normal that dim staining can be
observed in some somatic cells such as CHO cells, HeLa cells and cells from the placenta, and so on.
• After removal of dye from the media, fluorescently labeled cells lose their signal by exocytosis in 1.5-2 hours, please observe the
results within 1 hour after staining.
• Please avoid repeated freezing and thawing. When necessary, the working concentration can be increased.
Store at -20℃ in a dark place. Avoid repeated freezing and thawing
AP Live Solution (500×)