PerfectStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature. As the denaturation step progresses, the antibodies are inactivated and Taq DNA Polymerase is activated, which can effectively enhance the amplification efficiency and improve the amplification sensitivity and specificity. The amplified product has an "A" base at the 3' end and can be directly cloned into the pEASY®-T vectors.
• Compared with TransStart® Taq DNA Polymerase and TransStart® Top Taq DNA Polymerase, PerfectStart® Taq DNA Polymerase has higher amplification efficiency, specificity and sensitivity.
• Prepared at room temperature, reduced nonspecific and primer dimer formation.
• Different from chemical modification, long denaturing step is not needed.
• The reaction solution is specifically designed for molecular diagnosis, and the amplification length is no more than 1 kb.
• Hot start and high specificity.
• High sensitivity.
• High amplification efficiency.
• Low E. coli genomic DNA residue.
Applications
• Low copy number templates.
• Complex templates.
• GC/AT-rich templates.
• Real-time Quantitative PCR (qPCR).
• Multiplex PCR.
• Single nucleotide polymorphisms ( SNPs)
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AP401-01/11 | AP401-02/12 | AP401-03/13 |
| PerfectStart® Taq DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 10×PerfectStart®Taq Buffer | 1.2 ml×1 | 1.2 ml×2 | 1.2 ml×12 |
| 2.5 mM dNTPs | -/800 μl×1 | -/800 μl×2 | -/1.2 ml×8 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml×1 | 1 ml×2 |
| 10×GC Enhancer | 200 μl×1 | 400 μl×1 | 1 ml×1 |